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hs4800 hybridisation station  (Tecan Systems)


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    Tecan Systems hs4800 hybridisation station
    Hs4800 Hybridisation Station, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hs4800+hybridisation+station/hs4800+hybridization+station/pmc03295665-260-17-16
    Average 90 stars, based on 1 article reviews
    hs4800 hybridisation station - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Evaluation of serum microRNA biomarkers for gastric cancer based on blood and tissue pools profiling: the importance of miR-21 and miR-331
    Article Snippet: The labelled RNA samples were hybridised to the miRCURY LNA microRNA Array 7th Gen (Exiqon) containing capture probes targeting all microRNAs of human origin registered in the miRBASE 19.0. .. The hybridisation was performed using a Tecan HS4800 hybridisation station (Tecan, Salzburg, Austria) according to the manufacturer’s protocol. .. After hybridisation, the microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies) and analysed with the ImaGene 9 software (BioDiscovery, El Segundo, CA, USA).

    Article Title: The immediate global responses of Aliivibrio salmonicida to iron limitations
    Article Snippet: .. The labelled samples were hybridized to “ Vibrio salmonicida V1.0.1 AROS” slides (Eurofins MWG Operon) at 42°C for 20 hours on a TECAN HS4800 hybridisation station, and microarray slides were subsequently washed, once in 0.1 × SSC/0.1% SDS for 5 min at 42°C, then once in 0.1 × SSC/0.1% SDS for 10 min at room temperature, and finally four times in 0.1 × SSC for 1 min at room temperature. .. Slides were scanned using a GenePix 4000D scanner (Axon Instruments Inc.) at 532 and 635 nm.

    Article Title: Global microRNA expression profiles in insulin target tissues in a spontaneous rat model of type 2 diabetes
    Article Snippet: This array contains four replicates of each of the 239 rat miRNA probes as well as spike-ins, negative controls and proprietary Exiqon miRNA probes. .. Hybridisation was performed according to the miRCURY LNA array manual using a Tecan HS4800 hybridisation station (Tecan, Männedorf, Germany). ..

    Article Title: Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida
    Article Snippet: Quality of extracted RNA was examined by NanoDrop, and presence of RNase or DNase activity was checked by incubating the RNA with 500 ng plasmid DNA for 1 h at 37°C, followed by inspection of degradation on a EtBr-stained 1% agarose gel. cDNA was constructed from 15 μg purified RNA using Aminoallyl cDNA labeling kit (Applied Biosystems) and CyDyeTM Post-Labeling Reactive Dye Pack (GE Healthcare). .. The labelled samples were hybridized to " Vibrio salmonicida V1.0.1 AROS" slides at 42°C on a TECAN HS4800 hybridisation station. ..

    Article Title: MiR-17-92 and miR-221/222 cluster members target KIT and ETV1 in human gastrointestinal stromal tumours
    Article Snippet: One microgram of total RNA was fluorescently labelled with Cy3 using the Kreatech ULS aRNA labelling kit (Kreatech Diagnostics, Amsterdam, The Netherlands) following the standard protocol. .. Labelled RNA was hybridised in a Tecan HS4800 hybridisation station to in-home spotted array slides ( Pothof et al , 2009 ) with locked nucleic acid (LNA)-modified oligonucleotide capture probes (Exiqon, Vedbaek, Denmark) capable of detecting 725 human miRNAs in duplicate. .. Slides were scanned using a Tecan LS reloaded scanner, and median spot intensity was determined using ImaGene software (BioDiscovery Inc., Hawthorne, CA, USA).

    Article Title: A miRNA signature associated with human metastatic medullary thyroid carcinoma
    Article Snippet: The Hy3-labelled samples and a Hy5labelled reference RNA sample were mixed pair-wise and hybridised to the miRCURY LNA array version 10.0 (Exiqon), which contains more than 1200 capture probes covering all human, mouse and rat miRNA sequences annotated and registered in the miRBASE version 12.0 at the Sanger Institute (http://microrna.sanger.ac.uk/ sequences/) and Exiqon miRPlus mature miRNAs. .. The hybridisation was performed according to the miRCURY LNA array manual using a Tecan HS4800 hybridisation station (Tecan, Grödig, Austria). ..

    Article Title: NRF2 regulates endothelial glycolysis and proliferation with miR-93 and mediates the effects of oxidized phospholipids on endothelial activation
    Article Snippet: The Hy3TM-labeled samples and a Hy5TM-labeled reference RNA sample were mixed pair-wise and hybridized to the miRCURYTM LNA array version 11.0 (Exiqon, Denmark), which contained capture probes targeting all miRNAs for human, mouse or rat registered in the miRBASE version 13.0 at the Sanger Institute. .. The hybridisation was performed according to the miRCURYTM LNA array manual using a Tecan HS4800 hybridisation station (Tecan, Austria). ..

    Article Title: MicroRNA profiling of benign and malignant pheochromocytomas identifies novel diagnostic and therapeutic targets
    Article Snippet: The Hy3-labelled samples and a Hy5-labelled reference RNA sample www.endocrinology-journals.org were mixed pairwise and hybridised to the miRCURY LNA array version 11.0 (Exiqon), which contains capture probes targeting all miRNAs for human, mouse or rat registered in the miRBase version 13.0 at the Sanger Institute. .. The hybridisation was performed according to the miRCURY LNA array manual using a Tecan HS4800 hybridisation station (Tecan, Grodig, Austria). ..

    Microarray:

    Article Title: The immediate global responses of Aliivibrio salmonicida to iron limitations
    Article Snippet: .. The labelled samples were hybridized to “ Vibrio salmonicida V1.0.1 AROS” slides (Eurofins MWG Operon) at 42°C for 20 hours on a TECAN HS4800 hybridisation station, and microarray slides were subsequently washed, once in 0.1 × SSC/0.1% SDS for 5 min at 42°C, then once in 0.1 × SSC/0.1% SDS for 10 min at room temperature, and finally four times in 0.1 × SSC for 1 min at room temperature. .. Slides were scanned using a GenePix 4000D scanner (Axon Instruments Inc.) at 532 and 635 nm.



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